American Society of Microbiology ABMM : ABMM

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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A neonate develops a vesicular rash, fever, and seizures within the first week of life. CSF analysis reveals lymphocytic pleocytosis and elevated protein. PCR of the CSF is positive for a double-stranded DNA virus.
The MOST likely causative agent is:

A) Herpes simplex virus (HSV)
B) Cytomegalovirus (CMV)
C) Varicella-zoster virus (VZV)
D) Human parvovirus B19

2. A clinical microbiology laboratory is validating a new multiplex PCR assay for the simultaneous detection of several gastrointestinal pathogens, including Salmonella, Shigella, and Campylobacter. To ensure the accuracy and reliability of the assay, it is crucial to include appropriate controls. Which of the following sets of controls would be MOST comprehensive?

A) Positive controls for each target organism and a negative control without any template DNA.
B) Positive controls for each target organism, a negative control without template DNA, an internal amplification control, and a process control to monitor nucleic acid extraction.
C) Positive controls for each target organism, a negative control containing non-target DNA, and a process control to monitor nucleic acid extraction.
D) Positive controls for each target organism, a negative control without template DNA, and an internal amplification control.

3. A clinical microbiology laboratory is using Sanger sequencing to identify mutations associated with antibiotic resistance in Mycobacterium tuberculosis. This method directly determines the:

A) Minimum inhibitory concentration (MIC) of the antibiotic.
B) Nucleotide sequence of the target gene.
C) Presence or absence of specific resistance genes.
D) Relative abundance of different M. tuberculosis strains in a mixed infection.

4. A patient with a history of neutropenia develops a severe sinus infection. Tissue biopsy reveals broad, ribbon- like, non-septate hyphae with right-angle branching. The MOST likely causative organism belongs to the order:

A) Mucorales
B) Aspergillales
C) Eurotiales
D) Onygenales

5. A microbiology laboratory is investigating a possible outbreak of Salmonella infection. Serological testing of patient isolates reveals they all possess the same O antigen but different H antigens. This suggests:

A) A common ancestral strain that has undergone flagellar antigen variation.
B) A single point source of infection with a genetically stable strain.
C) Contamination in the laboratory affecting multiple samples.
D) Multiple sources of infection with unrelated Salmonella strains.

Solutions: